7JZ8

Dihydrodipicolinate synthase mutant S48F with lysine in the allosteric site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	294.15
Detector technology	CCD
Collection date	2011-11-10
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.95369
Spacegroup name	P 31 2 1
Unit cell lengths	121.049, 121.049, 110.151
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.310 - 1.820
R-factor	0.14468
Rwork	0.143
R-free	0.16984
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yxc
RMSD bond length	0.024
RMSD bond angle	2.168
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.300	1.860
High resolution limit [Å]	1.820	1.820
Rmerge	0.137
Number of reflections	83378	4541
<I/σ(I)>	11.7
Completeness [%]	100.0
Redundancy	10.8
CC(1/2)		0.846

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		249.15	glucopyrdoside, Dipotassium phosphate