7JZ5

Cellular retinol-binding protein 2 (CRBP2) in complex with 1-arachodonoyl-1-thio-glycerol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-07-14
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	57.816, 67.562, 89.477
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.918 - 1.567
R-factor	0.161702646311
Rwork	0.161
R-free	0.18309
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bti
RMSD bond length	0.008
RMSD bond angle	1.034
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.480	1.590
High resolution limit [Å]	1.567	1.570
Rmerge	0.089	0.689
Rmeas	0.111	0.818
Rpim	0.055	0.432
Number of reflections	49723	2289
<I/σ(I)>	14.1	2.1
Completeness [%]	99.6	93.8
Redundancy	6.5
CC(1/2)	0.994	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	0.1 M Tris HCl, PEG 3350 22-28%