7JYX
Crystal Structure of HLA A*2402 in complex with TYQWIIRNWET, an 11-mer epitope from Influenza
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 89.678, 43.645, 236.491 |
| Unit cell angles | 90.00, 95.56, 90.00 |
Refinement procedure
| Resolution | 39.210 - 2.950 |
| R-factor | 0.212 |
| Rwork | 0.208 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f7m |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.230 | 3.130 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.091 | 0.404 |
| Number of reflections | 19624 | 3149 |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 99.6 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.995 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 24% PEG8000, 0.1 HEPES pH 7.5, 5% v/v Ethyl acetate |
