7JXR

Crystal Structure Human Immunodeficiency Virus-1 Matrix protein Mutant Q63R Crystal Form 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	293
Detector technology	PIXEL
Collection date	2019-12-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	63.136, 97.923, 84.817
Unit cell angles	90.00, 99.94, 90.00

Refinement procedure

Resolution	46.210 - 2.040
R-factor	0.2042
Rwork	0.203
R-free	0.23390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hiw
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.210	2.113
High resolution limit [Å]	2.040	2.040
Rmerge	0.067	0.408
Rmeas	0.083	0.508
Rpim	0.048	0.298
Number of reflections	61646	6078
<I/σ(I)>	28.86	2.5
Completeness [%]	95.0
Redundancy	2.8
CC(1/2)	0.987	0.726

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4	293	40 % PEG 400, 0.1 M NaAcetate (pH 4.0), 0.05 M Li2SO4