7JX2

Cellular retinol-binding protein 2 (CRBP2) in complex with 2-palmitoylglycerol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-03-13
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	37.339, 64.450, 66.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.310 - 1.800
R-factor	0.208
Rwork	0.206
R-free	0.25550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bti
RMSD bond length	0.008
RMSD bond angle	0.789
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.580	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.113	0.873
Rmeas	0.134
Rpim	0.072	0.554
Number of reflections	15466	889
<I/σ(I)>	8.2	2.3
Completeness [%]	99.7	100
Redundancy	6.3	6.6
CC(1/2)	0.996	0.881

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	0.1 M Tris HCl, PEG 3350 22-28%