7JX0
NVS-PI3-4 bound to the PI3Kg catalytic subunit p110 gamma
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-08-24 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 143.620, 67.560, 106.830 |
| Unit cell angles | 90.00, 95.41, 90.00 |
Refinement procedure
| Resolution | 44.736 - 3.150 |
| R-factor | 0.2309 |
| Rwork | 0.229 |
| R-free | 0.27220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jha |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.736 | 3.260 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.119 | 1.118 |
| Number of reflections | 17573 | 1761 |
| <I/σ(I)> | 7.92 | 0.84 |
| Completeness [%] | 98.1 | 99.04 |
| Redundancy | 3 | |
| CC(1/2) | 0.994 | 0.425 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 100 mM Tris pH 7.5, 250 mM (NH4)2SO4 and 20-22% PEG 4000 |
