7JW6

Crystal structure of Drosophila Nibbler EXO domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-08
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9792
Spacegroup name	C 2 2 21
Unit cell lengths	44.338, 117.101, 87.481
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.300 - 1.500
R-factor	0.2032
Rwork	0.202
R-free	0.23170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	model from SAD
RMSD bond length	0.005
RMSD bond angle	0.754
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18_3855)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.300	1.554
High resolution limit [Å]	1.500	1.500
Rmerge	0.168	1.262
Rmeas	0.175	1.313
Rpim	0.048	0.358
Number of reflections	36858	3620
<I/σ(I)>	15.06	2.2
Completeness [%]	97.5	99.94
Redundancy	13.1	13.1
CC(1/2)	0.995	0.713

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.064 M sodium citrate 7.0, 0.1 M HEPES, pH 7.0, 10% PEG5000MME