7JVS

Crystal Structure of an Essential Ribosomal Processing Protease Prp from S. aureus in complex with a Substrate Peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-17
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54056
Spacegroup name	C 2 2 21
Unit cell lengths	52.552, 56.824, 130.278
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.840 - 2.300
R-factor	0.2266
Rwork	0.224
R-free	0.26410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4peo
RMSD bond length	0.017
RMSD bond angle	1.536
Data reduction software	CrysalisPro
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.840	28.840	2.390
High resolution limit [Å]	2.300	8.610	2.300
Rmerge	0.180	0.084	1.370
Rmeas	0.185	0.087	1.416
Rpim	0.043	0.022	0.353
Number of reflections	9024	205	941
<I/σ(I)>	10.8	24.8	2.1
Completeness [%]	100.0	98	100
Redundancy	18.6	14.5	15.8
CC(1/2)	0.998	0.998	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	PEG 1000, Hepes, CaCl2