7JUO
CBP bromodomain complexed with YF2-23
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-02-26 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97872 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 175.956, 70.132, 107.261 |
| Unit cell angles | 90.00, 120.37, 90.00 |
Refinement procedure
| Resolution | 19.940 - 2.200 |
| R-factor | 0.2138 |
| Rwork | 0.210 |
| R-free | 0.27580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ktu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.569 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.940 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.068 | 0.961 |
| Number of reflections | 57159 | 4291 |
| <I/σ(I)> | 9 | 1.4 |
| Completeness [%] | 99.4 | 95.9 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 277 | 0.2 M lithium citrate tribasic tetrahydrate, 17-19% PEG 3350, pH 8.4 |
