7JU2

Crystal structure of the monomeric ETV6 PNT domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-08-31
Detector	RAYONIX MX300HE
Wavelength(s)	0.9795
Spacegroup name	P 65 2 2
Unit cell lengths	59.860, 59.860, 169.380
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.215 - 1.850
R-factor	0.201380319359
Rwork	0.200
R-free	0.23391
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lky
RMSD bond length	0.006
RMSD bond angle	0.731
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.11.1-2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.215	44.215	1.900
High resolution limit [Å]	1.850	8.270	1.850
Rmerge	0.075	0.033	1.266
Rmeas	0.077	0.035	1.293
Number of reflections	16167	238	1150
<I/σ(I)>	28.24	70.66	2.79
Completeness [%]	100.0	99.2	100
Redundancy	22.966	16.647	23.636
CC(1/2)	0.999	0.998	0.901

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298.15	2.8 M Sodium Acetate