7JTU

Cytidine deaminase T6S toxin from Pseudomonas syringae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	80
Detector technology	CCD
Collection date	2020-02-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9790
Spacegroup name	I 41 2 2
Unit cell lengths	93.069, 93.069, 383.492
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.390 - 3.000
R-factor	0.2054
Rwork	0.202
R-free	0.23480
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	3.030
High resolution limit [Å]	3.000	3.000
Rmerge	0.120
Rpim		0.279
Number of reflections	17597	434
<I/σ(I)>	22.9
Completeness [%]	100.0	100
Redundancy	28.6	29.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Hanging drop diffusion. SsdA/SsdAI complex at 10 mg/mL was mixed 1:1 with crystallization solutions containing 20% PEG 3350, 0.1 M Bis-Tris:HCl pH 7.5 and 200 mM MgCl2. Cryopreservation was in crystallization solution with 20% glycerol.