7JTG

Structure of Hepatitis C Virus Envelope Glycoprotein E2 core from genotype 6a bound to broadly neutralizing antibody RM11-43

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-18
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	59.956, 65.343, 215.871
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.750 - 2.600
R-factor	0.2248
Rwork	0.222
R-free	0.26920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bkb
RMSD bond length	0.004
RMSD bond angle	0.804
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.640
High resolution limit [Å]	2.600	2.600
Number of reflections	26778	1146
<I/σ(I)>	14.8
Completeness [%]	99.2
Redundancy	7
CC(1/2)	0.920	0.630

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6	293	20% (w/v) PEG 3350, 0.2M sodium sulfate, pH 6.6