7JS4
The structure of the M60 catalytic domain with the CBM51-1 and CBM51-2 domains from Clostridium perfringens ZmpB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-13 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54187 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.908, 119.321, 129.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.690 - 4.600 |
| R-factor | 0.1945 |
| Rwork | 0.191 |
| R-free | 0.25820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5kdn |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.563 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 4.680 |
| High resolution limit [Å] | 4.600 | 4.600 |
| Rmerge | 0.204 | 0.741 |
| Rpim | 0.092 | 0.348 |
| Number of reflections | 6474 | 312 |
| <I/σ(I)> | 8.7 | 2.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.8 | 4.8 |
| CC(1/2) | 0.977 | 0.849 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 291 | 0.2 M potassium citrate tribasic monohydrate, 20% PEG3350 |
