7JRT

Crystal structures of artificially designed homomeric RNA nanoarchitectures

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-12
Detector	PSI PILATUS 6M
Wavelength(s)	1.10480
Spacegroup name	P 1 21 1
Unit cell lengths	86.436, 71.990, 89.439
Unit cell angles	90.00, 117.61, 90.00

Refinement procedure

Resolution	45.530 - 3.070
R-factor	0.1924
Rwork	0.189
R-free	0.22680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID: 2NOK
RMSD bond length	0.006
RMSD bond angle	1.220
Data reduction software	HKL-2000 (718)
Data scaling software	HKL-2000 (718)
Phasing software	PHENIX (1.10.1_2155)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.530	3.180
High resolution limit [Å]	3.070	3.070
Rmerge	0.100	0.510
Number of reflections	18129	1817
<I/σ(I)>	39.2
Completeness [%]	99.3
Redundancy	16.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	295	80 mM NaCl, 12 mM KCl, 20 mM MgCl2, 40 mM sodium cacodylate pH 6.0, 30 % (v/v) MPD, 12 mM spermine tetrahydrochloride