7JRS
Crystal structures of artificially designed homomeric RNA nanoarchitectures
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-11 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.979180 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.623, 64.795, 69.578 |
| Unit cell angles | 85.70, 64.25, 79.91 |
Refinement procedure
| Resolution | 38.289 - 3.210 |
| R-factor | 0.2768 |
| Rwork | 0.275 |
| R-free | 0.29660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID: 4CS1 |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.318 |
| Data reduction software | HKL-2000 (718) |
| Data scaling software | HKL-2000 (718) |
| Phasing software | PHENIX (1.11.1_2575) |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.270 |
| High resolution limit [Å] | 3.210 | 3.210 |
| Rmerge | 0.069 | 0.804 |
| Number of reflections | 15105 | 790 |
| <I/σ(I)> | 29.6 | |
| Completeness [%] | 97.8 | 95.9 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 20 mM MgCl2, 0.3 mM spermine tetrahydrochloride, 50 mM sodium succinate pH 5.5, 3.0 M Ammonium Sulfate |
