7JQY
Crystal structure of Cfl1-D123S from Burkholderia cenocepacia
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-09-20 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9201 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 195.000, 98.400, 170.000 |
Unit cell angles | 90.00, 118.60, 90.00 |
Refinement procedure
Resolution | 44.710 - 2.150 |
R-factor | 0.2104 |
Rwork | 0.209 |
R-free | 0.23970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kd2 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.367 | 2.280 |
High resolution limit [Å] | 2.150 | 2.150 |
Number of reflections | 152155 | 23995 |
<I/σ(I)> | 10.96 | |
Completeness [%] | 99.3 | |
Redundancy | 3.5 | |
CC(1/2) | 0.998 | 0.781 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 6% ethylene glycol, 8% PEG 8000, 100 mM HEPES pH 7.6 |