7JPI

Crystal structure of EBOV glycoprotein with modified HR2 stalk at 2.3A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	H 3 2
Unit cell lengths	114.583, 114.583, 312.379
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.000 - 2.280
R-factor	0.2015
Rwork	0.200
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jq3
RMSD bond length	0.002
RMSD bond angle	0.528
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.050	2.340
High resolution limit [Å]	2.280	2.280
Number of reflections	34904	1725
<I/σ(I)>	8	0.9
Completeness [%]	96.4	97.3
Redundancy	4.5
CC(1/2)	0.781	0.325

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	0.M Sodium citrate, 10% PEG w/v 6000