7JNH

Crystal structure of a double-ENE RNA stability element in complex with a 28-mer poly(A) RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9272
Spacegroup name	P 64 2 2
Unit cell lengths	93.973, 93.973, 202.171
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.030 - 2.890
R-factor	0.1862
Rwork	0.184
R-free	0.22540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	dENE
RMSD bond length	0.008
RMSD bond angle	1.951
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.000
High resolution limit [Å]	2.890	7.730	2.890
Rmerge	0.147	0.047	3.293
Rmeas	0.151	0.049	3.450
Rpim	0.037	0.013	0.979
Number of reflections	11859	776	812
<I/σ(I)>	17.05		0.66
Completeness [%]	95.1	99.4
Redundancy	16.4	15.9	10.2
CC(1/2)	0.994	0.998	0.199

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	50 mM sodium cacodylate, pH 7.0, 9 mM MgCl2, 1.8 mM cobalt (III) hexamine chloride, 0.9 mM spermidine, 2.5 mM spermine and 5% PEG 400