7JND

The structure of CBM32-1 and CBM32-2 domains from Clostridium perfringens ZmpB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-11
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91966
Spacegroup name	P 21 21 21
Unit cell lengths	43.260, 81.970, 132.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.160 - 2.000
R-factor	0.2369
Rwork	0.235
R-free	0.27920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jnb
RMSD bond length	0.006
RMSD bond angle	1.378
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.530	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.168	0.695
Rpim	0.069	0.285
Number of reflections	31552	4198
<I/σ(I)>	6.4	2.5
Completeness [%]	96.5	89.9
Redundancy	6.5	6.6
CC(1/2)	0.988	0.756

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0.18 M calcium chloride, 20% PEG3350, 0.1 M HEPES