7JMS

Structure of the Hazara virus OTU bound to ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-02
Detector	RAYONIX MX-225
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	83.850, 55.595, 97.434
Unit cell angles	90.00, 97.20, 90.00

Refinement procedure

Resolution	41.590 - 2.780
R-factor	0.2367
Rwork	0.235
R-free	0.27310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3prp 4hxd 5jze 6oar
RMSD bond length	0.006
RMSD bond angle	0.950
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.830
High resolution limit [Å]	2.780	2.780
Number of reflections	22676	1993
<I/σ(I)>	10.84	2.4
Completeness [%]	96.9
Redundancy	4.7
CC(1/2)	0.990	0.641

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.3 M calcium chloride, 20% PEG 4000