7JM5

Crystal structure of KDM4B in complex with QC6352

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	59.825, 97.107, 80.068
Unit cell angles	90.00, 106.52, 90.00

Refinement procedure

Resolution	41.030 - 2.700
R-factor	0.198
Rwork	0.196
R-free	0.24370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lxl
RMSD bond length	0.002
RMSD bond angle	0.476
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.760
High resolution limit [Å]	2.700	2.700
Rmerge	0.125	0.763
Rpim	0.051	0.340
Number of reflections	24351	1435
<I/σ(I)>	16.5	1.6
Completeness [%]	99.2	93.4
Redundancy	6.6	5.4
CC(1/2)	0.993	0.867

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	tri-sodium citrate, PEG 3350