7JM1
Crystal structure of aminoglycoside resistance enzyme ApmA, complex with acetyl-CoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.207, 77.233, 97.436 |
| Unit cell angles | 90.00, 102.52, 90.00 |
Refinement procedure
| Resolution | 37.840 - 2.310 |
| R-factor | 0.1586 |
| Rwork | 0.157 |
| R-free | 0.19720 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.463 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15_3448) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.050 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.046 | 0.296 |
| Rpim | 0.030 | 0.214 |
| Number of reflections | 42341 | 2929 |
| <I/σ(I)> | 17.6 | 2.96 |
| Completeness [%] | 96.9 | 92.4 |
| Redundancy | 2.9 | |
| CC(1/2) | 0.862 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1M Citric Acid pH3.6, 30% PEG 200, 5mM Apramycin, 2mM AcCoA |
