7JLT

Crystal Structure of SARS-CoV-2 NSP7-NSP8 complex.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9774
Spacegroup name	P 1 21 1
Unit cell lengths	42.828, 110.566, 42.904
Unit cell angles	90.00, 105.73, 90.00

Refinement procedure

Resolution	41.300 - 2.700
R-factor	0.2411
Rwork	0.236
R-free	0.28300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ahm
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (dev_3908)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.800
High resolution limit [Å]	2.700	5.810	2.700
Rmerge	0.129	0.062	0.827
Rmeas	0.149	0.071	0.955
Rpim	0.073	0.034	0.469
Total number of observations	41299
Number of reflections	10428	1081	1037
<I/σ(I)>	4.8
Completeness [%]	99.3	100	99.8
Redundancy	4	4.4	3.9
CC(1/2)		0.995	0.627

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	0.2 M Magnesium Chloride Hexahydrate, 0.1 M Bis-Tris pH 6.5, 25% w/v PEG 3350