7JLN

Crystal structure of glVRC01 Fab in complex with anti-idiotype iv9 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	69.420, 50.073, 125.171
Unit cell angles	90.00, 93.10, 90.00

Refinement procedure

Resolution	47.720 - 2.570
R-factor	0.1998
Rwork	0.197
R-free	0.25570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mft
RMSD bond length	0.003
RMSD bond angle	0.582
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.665
High resolution limit [Å]	2.570	2.573
Number of reflections	26793	2057
<I/σ(I)>	7.92
Completeness [%]	99.8
Redundancy	3.2
CC(1/2)	0.981	0.676

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.08 M Sodium Acetate, 24% PEG 4000, 0.16 M Ammonium Acetate