7JLK

Crystal structure of glVRC01 Fab in complex with anti-idiotype iv1 scFv

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-20
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.2
Spacegroup name	C 1 2 1
Unit cell lengths	70.019, 85.244, 113.636
Unit cell angles	90.00, 99.73, 90.00

Refinement procedure

Resolution	42.620 - 1.990
R-factor	0.182
Rwork	0.179
R-free	0.23540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mft
RMSD bond length	0.002
RMSD bond angle	0.506
Data scaling software	HKL-2000
Phasing software	PHASER (1.15.2_3472)
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	1.990	1.990
Number of reflections	44594	4230
<I/σ(I)>	4
Completeness [%]	99.2
Redundancy	1.7
CC(1/2)	0.911	0.822

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Hepes pH 7.0, PEG 8000