7JKM
REGN1 Human Fab in complex with anti-Kappa VHH domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-10 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | .91942 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.365, 75.930, 64.003 |
| Unit cell angles | 90.00, 90.81, 90.00 |
Refinement procedure
| Resolution | 29.490 - 1.780 |
| R-factor | 0.1833 |
| Rwork | 0.182 |
| R-free | 0.21030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ioi |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.530 |
| Data reduction software | DIALS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.490 | 1.847 |
| High resolution limit [Å] | 1.780 | 1.783 |
| Rmerge | 0.025 | 0.329 |
| Rmeas | 0.036 | 0.465 |
| Rpim | 0.025 | 0.329 |
| Number of reflections | 53611 | 3714 |
| <I/σ(I)> | 16.21 | 1.52 |
| Completeness [%] | 94.1 | 65.64 |
| Redundancy | 1.9 | 1.7 |
| CC(1/2) | 0.999 | 0.866 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 25.5% (w/v) PEG 4000, 0.17M ammonium acetate, 0.085M sodium citrate HCl, pH 5.6, with 15% (v/v) glycerol |
