7JJF

Sarcin-ricin loop with modified residue.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	LIQUID ANODE
Source details	Excillum MetalJet D2+ 70 kV
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-19
Detector	Bruker PHOTON III
Wavelength(s)	1.3418
Spacegroup name	P 43
Unit cell lengths	29.340, 29.340, 75.661
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.360 - 1.200
R-factor	0.184
Rwork	0.177
R-free	0.21400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dvz
RMSD bond length	0.007
RMSD bond angle	1.342
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.360	1.240
High resolution limit [Å]	1.200	1.200
Rmerge	0.089
<I/σ(I)>	23.47
Completeness [%]	94.1
Redundancy	17

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	RNA was dissolved in 1 mM Na2 EDTA (pH 8.0) and 10 mM Tris-HCl (pH 8.0) to attain 350 uM final RNA concentration. The RNA sample was annealed by heating at 65 C for 2 min and slowly cooling to room temperature. Crystallization set-ups were made by mixing 4 uL of RNA solution with 2 uL of a crystallization buffer composed of 3.0 M ammonium sulfate, 10 mM magnesium chloride, 10 mM manganese chloride, and 50 mM potassium 3-(N-morpholino) propanesulfonic acid (MOPS), pH 7.0 at 18 C. Crystals appear in about two weeks time.