7JJD

Sarcin-ricin loop with guanosine monothiophosphate residue.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2018-04-10
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 43
Unit cell lengths	29.639, 29.639, 76.658
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.210
Rwork	0.173
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID: 3S7C
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	SHELX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.250
High resolution limit [Å]	1.210	2.610	1.210
Rmerge	0.055	0.034	0.538
Rmeas	0.062	0.038	0.629
Rpim	0.028	0.017	0.320
Total number of observations	91157
Number of reflections	19854	2038	1912
<I/σ(I)>	11.7
Completeness [%]	99.2	99.6	95.6
Redundancy	4.6	5	3.6
CC(1/2)		0.999	0.764

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Crystallization set-ups were made by mixing 4 uL of 350 uM RNA solution [1 mM Na 2 EDTA (pH 8.0) and 10 mM Tris-HCl (pH 8.0)] with 2 uL of a crystallization buffer composed of 3.0 M ammonium sulfate, 10 mM magnesium chloride, 10 mM manganese chloride, and 50 mM potassium 3-(N-morpholino) propanesulfonic acid (MOPS), pH 7.0 at 18 C.