7JIK

Human recombinant Beta-2-Glycoprotein 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	C 2 2 21
Unit cell lengths	159.645, 172.976, 113.439
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.930 - 2.690
R-factor	0.2218
Rwork	0.221
R-free	0.23830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qub
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.790
High resolution limit [Å]	2.690	5.790	2.690
Rmerge	0.216	0.092	3.108
Rmeas	0.231	0.099	3.301
Rpim	0.080	0.035	1.102
Total number of observations	365257
Number of reflections	43781	4577	4300
<I/σ(I)>	7
Completeness [%]	99.7	99.5	100
Redundancy	8.3	8	8.8
CC(1/2)		0.993	0.281

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	100mM Hepes, pH 7.5, 1.8M Ammonium Sulfate