7JID
Crystal structure of the L780 UDP-rhamnose synthase from Acanthamoeba polyphaga mimivirus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-24 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.803, 52.989, 70.149 |
| Unit cell angles | 81.94, 88.68, 66.74 |
Refinement procedure
| Resolution | 30.300 - 1.450 |
| R-factor | 0.1992 |
| Rwork | 0.198 |
| R-free | 0.22680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qqr |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.853 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 110458 | 18606 |
| <I/σ(I)> | 13.5 | 2.7 |
| Completeness [%] | 99.5 | 88.7 |
| Redundancy | 4.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 20% PEG-8000, 2% DMSO, 100 mM CHES, 5 mM NADP(+), 5 mM UDP-L-rhamnose, 200 mM NaCl |
