7JHI
Structure of human beta 1,3-N-acetylglucosaminyltransferase 2 iodide-derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 87 |
| Detector technology | PIXEL |
| Collection date | 2017-09-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.85 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.431, 81.385, 157.138 |
| Unit cell angles | 90.00, 98.63, 90.00 |
Refinement procedure
| Resolution | 47.330 - 2.500 |
| R-factor | 0.2039 |
| Rwork | 0.202 |
| R-free | 0.24760 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.592 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.29) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.330 | 47.330 | 2.570 |
| High resolution limit [Å] | 2.500 | 10.900 | 2.500 |
| Rmerge | 0.146 | 0.049 | 1.360 |
| Rmeas | 0.152 | 0.051 | 1.418 |
| Rpim | 0.042 | 0.014 | 0.397 |
| Total number of observations | 772620 | 9070 | 56383 |
| Number of reflections | 58434 | 752 | 4497 |
| <I/σ(I)> | 17.7 | 71.6 | 2 |
| Completeness [%] | 100.0 | 98.8 | 100 |
| Redundancy | 13.2 | 12.1 | 12.5 |
| CC(1/2) | 0.998 | 0.999 | 0.753 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 24% PEG1500, 20% glycerol |
