7JH3
Crystal structure of 4-aminobutyrate aminotransferase PuuE from Escherichia coli in complex with PLP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-09-29 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 198.641, 78.839, 164.976 |
| Unit cell angles | 90.00, 112.82, 90.00 |
Refinement procedure
| Resolution | 24.880 - 2.680 |
| R-factor | 0.1794 |
| Rwork | 0.178 |
| R-free | 0.21130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6wj8 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.712 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.730 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Rpim | 0.051 | 0.235 |
| Number of reflections | 67076 | 3334 |
| <I/σ(I)> | 12.1 | 2 |
| Completeness [%] | 98.7 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 0.1M di-Na Tartrate, 0.1M Tris pH8.5, 20%PEG3350, 2.2mM PLP, 1%Et.Glycol |
