7JGT

Crystal Structure of FN3tt

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2007-05-18
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	38.530, 38.530, 105.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.110 - 1.900
R-factor	0.1826
Rwork	0.177
R-free	0.23350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Rosetta generated
Data reduction software	DENZO
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18_3855)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	31.110	31.110	1.950
High resolution limit [Å]	1.900	8.500	1.900
Rmerge	0.064	0.039	0.322
Rmeas	0.065	0.040	0.333
Total number of observations	161213
Number of reflections	6681	105	400
<I/σ(I)>	38.63	71.28	8.85
Completeness [%]	98.3	99.1	79.7
Redundancy	24.13	18.524	14.538
CC(1/2)	1.000	0.999	0.965

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	298	0.1 M MES pH 6.5, 28% PEG 3350