7JFP

GTP-specific succinyl-CoA synthetase complexed with Mg-GDP, phosphohistidine loop pointing towards nucleotide binding site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00000
Spacegroup name	P 32 2 1
Unit cell lengths	99.645, 99.645, 134.262
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	52.990 - 2.550
R-factor	0.2423
Rwork	0.240
R-free	0.29300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fp4
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (dev_3885)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.990	2.590
High resolution limit [Å]	2.550	2.550
Rmerge	0.084
Rpim	0.063
Number of reflections	25660	1278
<I/σ(I)>	8.1	0.2
Completeness [%]	99.8
Redundancy	4.6
CC(1/2)	0.999	0.297

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	1.0 M sodium citrate, Tris-HCl pH 7.4