7FZG

Crystal Structure of apo mouse FABP4, C-centered orthorhombic form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-08-21
Detector	PSI PILATUS 6M
Wavelength(s)	0.999980
Spacegroup name	C 2 2 21
Unit cell lengths	77.801, 94.742, 49.864
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.370 - 1.490
R-factor	0.1441
Rwork	0.143
R-free	0.17020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	inhouse model
RMSD bond length	0.019
RMSD bond angle	2.008
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.7.0027)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.370	47.370	1.530
High resolution limit [Å]	1.490	6.660	1.490
Rmerge	0.033	0.018	1.519
Rmeas	0.039	0.020	1.646
Total number of observations	198463
Number of reflections	30207	398	2180
<I/σ(I)>	21.92	73.59	1.43
Completeness [%]	98.9	99	97.3
Redundancy	6.54	5.872	6.692
CC(1/2)	1.000	1.000	0.702

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	protein in 25mM Tris/HCl pH 7.5 100mM NaCl, see also PMID 27658368