7FTK
Crystal Structure of apo human cyclic GMP-AMP synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-05 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.999770 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.128, 120.413, 127.506 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.207 - 2.125 |
R-factor | 0.2154 |
Rwork | 0.212 |
R-free | 0.27810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | inhouse model |
RMSD bond length | 0.008 |
RMSD bond angle | 0.929 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.12rc1_2801) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 60.210 | 60.207 | 2.180 |
High resolution limit [Å] | 2.130 | 9.500 | 2.130 |
Rmerge | 0.056 | 0.022 | 1.904 |
Rmeas | 0.060 | 0.024 | 2.046 |
Total number of observations | 323700 | ||
Number of reflections | 44441 | 600 | 3268 |
<I/σ(I)> | 15.74 | 46.35 | 0.99 |
Completeness [%] | 99.9 | 99.2 | 100 |
Redundancy | 7.284 | 6.217 | 7.56 |
CC(1/2) | 0.999 | 0.999 | 0.406 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 10-12 mg/mL protein in 25 mM Tris/HCl pH7.5, 500mM NaCl, 2mM TCEP, supplemented with 10x molar excess of ligand and, if needed, with 10 mM MgCl2 and 5mM ATP, then mixed 1:1 with reservoir of the Procomplex screen. Several conditions resulted in crystals. |