7FSE

Crystal Structure of T. maritima reverse gyrase with a minimal latch

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-28
Detector	EIGER 16M
Wavelength(s)	1.00000
Spacegroup name	P 41 21 2
Unit cell lengths	91.832, 91.832, 323.312
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.620 - 2.890
R-factor	0.2154
Rwork	0.213
R-free	0.26380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ddu
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	88.340	55.620	2.970
High resolution limit [Å]	2.890	12.920	2.890
Rmerge	0.298	0.053	4.917
Rmeas	0.309	0.056	5.090
Total number of observations	465336
Number of reflections	32090	442	2327
<I/σ(I)>	7.86	33.22	0.55
Completeness [%]	99.9	98.9	99.9
Redundancy	14.501	11.545	14.962
CC(1/2)	0.997	0.996	0.262

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.9	293	54 microM protein in 50mM Tris/HCl, pH7.5, 500mM NaCl, 10mM MgCl2, 100 microM ZnCl2, 2mM BME mixed 1:1 with 20% PEG3350 0.2 M KNO3, total volume 200nL