7FJC

Crystal structure of SARS-CoV-2 Beta RBD complexed with P36-5D2 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9798
Spacegroup name	C 1 2 1
Unit cell lengths	165.110, 42.113, 122.089
Unit cell angles	90.00, 102.79, 90.00

Refinement procedure

Resolution	43.480 - 2.960
R-factor	0.2146
Rwork	0.212
R-free	0.26910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m0j 6rcs 7k8r
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.480	3.070
High resolution limit [Å]	2.960	2.960
Number of reflections	16679	1235
<I/σ(I)>	11.1
Completeness [%]	94.7
Redundancy	6.4
CC(1/2)	0.958	0.451

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	0.1M Sodium HEPES 7.5, 10% w/v PEG6000, 5% v/v MPD