7FJ8

KpAckA (PduW) with AMP complex structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	C 2 2 21
Unit cell lengths	102.098, 152.635, 131.743
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.040 - 2.100
R-factor	0.178
Rwork	0.177
R-free	0.21460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7fj7
RMSD bond length	0.007
RMSD bond angle	0.821
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.100	2.100
Rpim	0.070	0.335
Number of reflections	60817	2928
<I/σ(I)>	27.68	3.41
Completeness [%]	99.3	97.9
Redundancy	12.1	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	0.1 M Tris,10% (w/v) PEG-8000, 0.2M MgCl2, pH 7.0.