7FJ7
KpAckA (PduW) native structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 151.865, 101.510, 90.794 |
| Unit cell angles | 90.00, 125.19, 90.00 |
Refinement procedure
| Resolution | 45.380 - 2.500 |
| R-factor | 0.1835 |
| Rwork | 0.181 |
| R-free | 0.23010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iir |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.939 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rpim | 0.032 | 0.211 |
| Number of reflections | 38499 | 1895 |
| <I/σ(I)> | 21.45 | 3.5 |
| Completeness [%] | 98.8 | 97.2 |
| Redundancy | 6.8 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 0.1 M BIS-TRIS, 45% v/v Polypropylene glycol P 400, pH 6.5. |
