7FJ2
Structure of FOXM1 homodimer bound to a palindromic DNA site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2020-12-20 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.58 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.387, 45.456, 133.591 |
| Unit cell angles | 90.00, 92.85, 90.00 |
Refinement procedure
| Resolution | 49.303 - 3.098 |
| R-factor | 0.2208 |
| Rwork | 0.216 |
| R-free | 0.26540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3g73 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.904 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.303 | 3.200 |
| High resolution limit [Å] | 3.098 | 3.100 |
| Number of reflections | 12865 | 810 |
| <I/σ(I)> | 10.1 | |
| Completeness [%] | 87.7 | |
| Redundancy | 5.2 | |
| CC(1/2) | 0.978 | 0.946 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 277 | 50 mM Sodium acetate pH 4.7 250 mM Sodium chloride 15% PEG 3350 |
