7FHW
Crystal structure of Multi-functional Polysaccharide lyase Smlt1473 from Stenotrophomonas maltophilia (strain K279a) in apo form at pH 5.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR-H |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2017-06-07 |
Detector | BRUKER PHOTON 100 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.272, 94.668, 160.408 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.204 - 2.061 |
Rwork | 0.207 |
R-free | 0.25480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7fhu |
RMSD bond length | 0.009 |
RMSD bond angle | 1.530 |
Data reduction software | PROTEUM PLUS |
Data scaling software | PROTEUM PLUS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.270 | 2.160 |
High resolution limit [Å] | 2.060 | 2.060 |
Rmerge | 0.119 | 0.434 |
Number of reflections | 45555 | 5473 |
<I/σ(I)> | 19.58 | 3.27 |
Completeness [%] | 96.2 | 89.8 |
Redundancy | 15.34 | 4.35 |
CC(1/2) | 0.996 | 0.750 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298.15 | 0.24M LiSo4 monohydrate, 100mM BIS-TRIS, pH5.5, PEG 3350 25%(w/v) |