7FHV
Crystal structure of Multi-functional Polysaccharide lyase Smlt1473 (WT) from Stenotrophomonas maltophilia (strain K279a) in apo form at pH 6.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2019-11-26 |
| Detector | BRUKER PHOTON 100 |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.513, 58.213, 67.543 |
| Unit cell angles | 71.33, 89.94, 74.86 |
Refinement procedure
| Resolution | 53.006 - 2.280 |
| Rwork | 0.196 |
| R-free | 0.24510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7fhu |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.429 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.020 | 2.380 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.082 | 0.338 |
| Number of reflections | 26004 | 3005 |
| <I/σ(I)> | 9.17 | 2.16 |
| Completeness [%] | 92.3 | 88.2 |
| Redundancy | 2.52 | 1.05 |
| CC(1/2) | 0.992 | 0.753 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298.15 | 0.2M LiSo4 monohydrate, 0.1M Sodium Cacodylate pH 6.5, 30% (w/v) PEG 8000 |
