7FHU

Crystal structure of Multi-functional Polysaccharide lyase Smlt1473 from Stenotrophomonas maltophilia (strain K279a) in apo form at pH 8.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR-H
Temperature [K]	100
Detector technology	CMOS
Collection date	2016-03-26
Detector	BRUKER PHOTON 100
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	44.022, 66.804, 103.120
Unit cell angles	90.00, 94.39, 90.00

Refinement procedure

Resolution	43.854 - 2.430
Rwork	0.227
R-free	0.27370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qaz
RMSD bond length	0.007
RMSD bond angle	1.227
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.890	2.530
High resolution limit [Å]	2.430	2.430
Rmerge	0.123	0.442
Number of reflections	22678	2531
<I/σ(I)>	19.97	5.11
Completeness [%]	100.0	100
Redundancy	17.66	11.4
CC(1/2)	0.998	0.948

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298.15	0.2M lithium sulfate monohydrate, 0.1M Tris-HCl pH-8.5, 30% (w/v) PEG 4000.