7FHD

Structure of prenyltransferase mutant Y288P from Streptomyces sp. (strain CL190)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-03
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.95373
Spacegroup name	P 1
Unit cell lengths	44.286, 75.162, 87.605
Unit cell angles	89.94, 89.90, 90.00

Refinement procedure

Resolution	29.200 - 3.500
R-factor	0.2466
Rwork	0.243
R-free	0.31300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zb6
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.200	3.830
High resolution limit [Å]	3.500	3.500
Rmerge	0.262	0.446
Rmeas	0.339	0.578
Rpim	0.211	0.362
Total number of observations	29344
Number of reflections	13654	3344
<I/σ(I)>	2.2
Completeness [%]	95.8	97.7
Redundancy	2.1	2.2
CC(1/2)	0.914	0.797

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	6% v/v Tacsimate pH 6.0, 0.1 M MES monohydrate pH 6.0, 25% w/v PEG 4,000