7FGR
The cross-reaction complex structure with VQIFNK peptide and the tau antibody's Fab domain.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2021-03-03 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 179.050, 70.140, 41.260 |
| Unit cell angles | 90.00, 93.15, 90.00 |
Refinement procedure
| Resolution | 32.670 - 2.200 |
| R-factor | 0.20355 |
| Rwork | 0.200 |
| R-free | 0.27180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6lra |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.667 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.670 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.120 | 0.840 |
| Number of reflections | 23945 | 23945 |
| <I/σ(I)> | 6.2 | |
| Completeness [%] | 95.7 | |
| Redundancy | 3.64 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 18% PEG 3350, 20% Glycerol, 0.2M Ammonium iodide, 10mM Tris-HCl |
