7FGP

Crystal structure of Aureimonas altamirenisis flavin-containing opine dehydrogenase (FAD-bound form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.970000
Spacegroup name	P 65 2 2
Unit cell lengths	105.727, 105.727, 261.837
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.018 - 1.470
R-factor	0.1742
Rwork	0.174
R-free	0.18530
Structure solution method	SAD
Data scaling software	Aimless (0.7.4)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.020	49.020	1.500
High resolution limit [Å]	1.470	8.050	1.470
Rmerge	0.635	0.327	2.115
Rmeas	0.640	0.329	2.139
Rpim	0.076	0.040	0.321
Total number of observations	10079189	71123	312964
Number of reflections	146819	1103	7175
<I/σ(I)>	10.1	18.1	2.3
Completeness [%]	100.0	99.6	100
Redundancy	68.7	64.5	43.6
CC(1/2)	0.987	0.987	0.842

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.1 M Lithium sulfate, 0.1 M Bis-tris pH 5.5, 24.5% PEG 3350