7FGM

The complex crystals structure of the FAF1 UBL1_L-Hsp70 NBD with ADP and phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	298
Detector technology	CCD
Collection date	2013-05-14
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000000
Spacegroup name	P 31 2 1
Unit cell lengths	103.335, 103.335, 127.093
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.763 - 2.200
R-factor	0.176
Rwork	0.174
R-free	0.20570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1s3x
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.280
High resolution limit [Å]	2.200	4.740	2.200
Rmerge	0.098	0.047	0.322
Total number of observations	656487
Number of reflections	40339	4236	3957
<I/σ(I)>	7
Completeness [%]	99.9	99.5	99.9
Redundancy	16.3	18.9	13.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	200 mM Imidazole-malate pH 8.5 12 % PEG 10,000