7FGJ
The cross-reaction complex structure with VQILNK peptide and the tau antibody's Fab domain.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2021-04-23 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 69.931, 179.704, 41.431 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.150 - 1.890 |
| R-factor | 0.19922 |
| Rwork | 0.196 |
| R-free | 0.26206 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6lra |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.603 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.150 | 1.960 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.078 | 0.401 |
| Number of reflections | 39868 | 39868 |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 93.1 | |
| Redundancy | 6.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 18% PEG 3350, 15% Glycerol, 0.2M Ammonium iodide, 10mM Tris-HCl |
