7FEX

Crystal structure of Sus scrofa Schlafen11 N-terminal domain (2.69 A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 61 2 2
Unit cell lengths	64.991, 64.991, 344.018
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.560 - 2.690
R-factor	0.2177
Rwork	0.215
R-free	0.25840
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHARP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.564	2.710
High resolution limit [Å]	2.690	2.690
Rmerge	0.143	1.150
Number of reflections	22498	3610
<I/σ(I)>	26.33
Completeness [%]	99.9	99.8
Redundancy	34.4	34.8
CC(1/2)	0.999	0.886

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0.1M HEPES pH7.5, 22%PEG3350, 0.15M Lithium sulfate